Within one day, Italian health authorities reported 642 new coronavirus infections.
The last time a higher number was registered was May, with 669 new infections. The peak of new daily recorded infections was in March, with more than 6,500.
The number of swabs taken rose by almost 20,000 on Tuesday, to 71,000 in a day, the ministry said. The regions which recorded the biggest rises were Lombardy (+91) and Emilia Romagna (+76).
The total number of cases recorded since the start of the pandemic is 255,278. With 7 more fatalities recorded on Tuesday, the death toll stands at 35,412.
Over the weekend, Italian authorities ordered the closure of nightclubs and tightened mask-wearing rules after the number of new cases detected in the country rose from 200-300 to 600 within one week.
Many of Italy's new cases have been attributed to holidaymakers - often Italians returning from abroad, health authorities said.